Thermo Fisher Scientific Cytoskeletal Actin Recombinant Rabbit Monoclonal Antibody (BLR057F)
Recombinant rabbit monoclonal antibody targeting cytoskeletal beta-actin. Validated for WB, IHC(P), and ICC/IF. Recognizes human and mouse actin. Supplied as purified liquid (10 µg/mL) for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 – 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 – 1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR057F |
| Immunogen | Peptide representing a region between residues 1 and 50 of human Actin Beta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 10 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 7.8–8.6, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein found in all human cell types and plays a key role in cell motility, structure, and integrity. Beta and gamma actins coexist in most cell types as components of the cytoskeleton. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin) in a two-stranded helical structure. Due to its ubiquitous expression, beta actin is commonly used as a loading control in protein detection assays such as Western blotting. Antibodies against beta-actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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