Thermo Fisher Scientific Cdc20 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Cdc20. Recombinant protein control fragment (Product #RP-103211). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CDC20,uniProtId:Q12834-1,ncbiNodeId:9606,antigenRange:106-185,antigenLength:499,antigenImageFileName:PA5-63103_CDC20_Q12834-1_Rabbit.svg,antigenImageFileNamePDP:PA5-63103_CDC20_Q12834-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2639559

Product Specific Information

Immunogen sequence: TPTKKEHQKA WALNLNGFDV EEAKILRLSG KPQNAPEGYQ NRLKVLYSQK ATPGSSRKTC RYIPSLPDRI LDAPEIRNDY

Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 98%.

Target Information

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.