Thermo Fisher Scientific Cdc20 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Cdc20 for WB, IHC(P), and ICC/IF applications. High specificity with recombinant protein immunogen. Supplied as liquid, unconjugated form, purified by antigen affinity chromatography. Ideal for research use in...
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 | View publications |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | View publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human Cdc20. Recombinant protein control fragment (Product #RP-103211). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2639559 |
Product Specific Information
Immunogen sequence:
TPTKKEHQKA WALNLNGFDV EEAKILRLSG KPQNAPEGYQ NRLKVLYSQK ATPGSSRKTC RYIPSLPDRI LDAPEIRNDY
Sequence identity to orthologs:
- Mouse: 99%
- Rat: 98%
Target Information
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex.
Regulated by MAD2L1: in metaphase, the MAD2L1-CDC20-APC/C ternary complex is inactive; in anaphase, the CDC20-APC/C binary complex is active in substrate degradation.
The CDC20-APC/C complex positively regulates synaptic vesicle clustering at the presynaptic membrane in postmitotic neurons.
CDC20-APC/C-induced degradation of NEUROD2 promotes presynaptic differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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