Thermo Fisher Scientific ID2 Monoclonal Antibody (ILCID2), PE-Cyanine7, eBioscience

Thermo Fisher Scientific ID2 Monoclonal Antibody (ILCID2), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test
Publications: View 2 publications

Product Specifications

Additional Formats

• APC
• eFluor 450
• PE
• Request custom conjugation

Product Specific Information

This ILCID2 monoclonal antibody recognizes mouse and human ID2.
Requires Foxp3/Transcription Factor Staining Buffer Set for optimal performance; performs poorly with standard IC Fixation & Permeabilization Buffer Set.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).

Refer to BestProtocols®: Protocol B for intracellular staining.

• Recommended ≤1.0 µg/test (100 µL final volume)
• Cell number: 10⁵–10⁸ cells/test (empirical determination)
• Titrate for optimal performance

Light Sensitivity: Tandem dye sensitive to photo-induced oxidation. Protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in dark.
Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green

Target Information

The inhibitor of DNA binding 2 (ID2) protein promotes development of several cell types including natural killer cells, erythroid precursors, and CD8⁺/CD4⁺ T cells.
Deficiency of ID2 enhances B-cell development.
ID2 associates with E proteins and other basic helix-loop-helix (HLH) family members, preventing DNA binding and transcription activation.
Activation of ID2 is critical for NK cell lineage development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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