Thermo Fisher Scientific EEA1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunoprecipitation (IP)

2-5 µg/mg lysate

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 175 and 225 of human early endosome antigen 1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EEA1,uniProtId:Q15075-1,ncbiNodeId:9606,antigenRange:175-225,antigenLength:1411,antigenImageFileName:A301-897A-M_EEA1_Q15075-1_Rabbit.svg,antigenImageFileNamePDP:A301-897A-M_EEA1_Q15075-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.