Thermo Fisher Scientific Anza T4 PNK Kit
DNA 및 올리고뉴클레오타이드의 5’ 인산화를 위한 T4 폴리뉴클레오타이드 키나제 키트. 빠른 15분 실온 반응으로 효율적 처리 가능. 다양한 버퍼 조건에서 사용 가능하며 클로닝용 DNA 준비에 적합. Anza Restriction Enzyme Cloning System 구성품.
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Invitrogen™ Anza™ T4 PNK Kit
The Invitrogen™ Anza™ T4 PNK (polynucleotide kinase) Kit is used to perform 5′-phosphorylation of DNA and oligonucleotides.
T4 PNK exhibits both 5′ polynucleotide kinase and 3′ phosphatase activity, catalyzing the transfer of the terminal phosphate of ATP to a 5′ hydroxyl group of a nucleic acid.
Application
When preparing DNA for the ligation step in cloning, either the insert DNA or the vector DNA should contain a 5′ phosphate.
Often, PCR-amplified DNA is treated with T4 PNK to phosphorylate the 5′ end for subsequent cloning ligation.
Benefits
- Fast 15-minute incubation at room temperature
- Phosphorylation can be performed with DNA or oligonucleotides in water, TE, or 1X Anza buffers
The Anza T4 PNK Kit is part of the Anza™ Restriction Enzyme Cloning System, unifying traditional cloning processes.
Other Anza DNA Modifying Enzymes include:
제품 사양
| 항목 | 내용 |
|---|---|
| 배송 조건 | Approved for shipment on Wet or Dry Ice |
| 효소 | Polynucleotide Kinase |
| 함께 사용 가능한 버퍼 | Anza™ 1X Buffer, Anza™ 10X T4 PNK Buffer |
| 수량 | 50 reactions |
| 제품 유형 | T4 PNK Kit |
| 제품 라인 | Anza™ |
| Unit Size | Each |
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