Thermo Fisher Scientific CD45 Monoclonal Antibody (CD45-2B11), eBioscience
인간 CD45 항원을 인식하는 마우스 단클론 항체로, 림프구 증식 및 분화 연구에 적합합니다. WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능하며, 고순도(>90%) 및 낮은 응집도(<10%)로 안정적인 결과를 제공합니다.
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Thermo Fisher Scientific CD45 Monoclonal Antibody (CD45-2B11), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | - |
| Immunohistochemistry (IHC) | - | 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL | 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 0.25 µg/test | 2 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD45-2B11 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865379 |
Additional Formats:
Product Specific Information
Description:
The CD45-2B11 monoclonal antibody reacts with human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except erythrocytes and platelets. Its cytoplasmic domain possesses tyrosine phosphatase activity, crucial for lymphocyte proliferation and differentiation. The 2B11 antibody is useful for identifying normal and neoplastic lymphoid cells.
Applications Reported:
Suitable for flow cytometric analysis, immunoblotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and immunocytochemistry.
Applications Tested:
- IHC (Paraffin): ≤10 µg/mL (high pH antigen retrieval)
- WB: 5 µg/mL
- Flow Cytometry: 0.25 µg/test on human peripheral blood cells
A test is defined as the amount (µg) of antibody used to stain a cell sample in 100 µL final volume. Cell number may vary from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45 (LCA, Leukocyte Common Antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, constituting ~10% of lymphocyte surface proteins. It is absent in non-hematopoietic tissues and cell lines. CD45 is essential for lymphocyte development and antigen signaling, regulating Src-family kinases and JAK kinase pathways. Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing transmembrane and cytoplasmic regions. CD45 plays roles in T/B-cell receptor signaling, cytokine signaling regulation, integrin-mediated survival, and apoptosis modulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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