Thermo Fisher Scientific B2M Monoclonal Antibody (OTI1A7), TrueMAB
인간 B2M 단백질을 인식하는 단클론 항체로 Western blot, IHC, ELISA 등에 사용 가능. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. 항원은 인간 B2M(aa 21-119) 재조합 단백질 기반. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 21–119 of human B2M produced in SF9 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Beta-2-microglobulin (B2M) is a 12–14 kDa protein that forms part of the MHC class I complex. It is expressed on various cell types including lymphocytes, monocytes, granulocytes, platelets, and epithelial cells, but absent on erythrocytes.
B2M is structurally similar to immunoglobulin constant regions and mainly composed of beta-pleated sheets. It participates in antigen processing, presentation, and TCR signaling pathways.
The B2M gene is located on chromosome 15, and mutations can cause hypercatabolic hypoproteinemia. B2M levels are used as a marker for renal function and HIV prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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