Thermo Fisher Scientific DBT Monoclonal Antibody (OTI1G2), TrueMAB
DBT 단백질을 인식하는 Mouse 모노클로날 항체로 Western blot과 유세포분석에 적합. HEK293T 세포에서 발현된 full-length human DBT 단백질로 면역화됨. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 사용 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G2 |
| Immunogen | Full length human recombinant protein of human DBT produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of branched-chain amino acids (isoleucine, leucine, valine).
This gene encodes the transacylase (E2) subunit of the BCKD complex. Mutations in this gene are associated with maple syrup urine disease, type 2.
Alternatively spliced transcript variants have been described, though their biological significance remains undetermined.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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