Invitrogen™

Click-IT™ O-GlcNAc Enzymatic Labeling System

The Click-iT™ O-GlcNAc Enzymatic Labeling System provides a highly sensitive and efficient method for the in-vitro modification of O-GlcNAc modified자세히 알아보기

The Click-iT™ O-GlcNAc Enzymatic Labeling System provides a highly sensitive and efficient method for the in-vitro modification of O-GlcNAc modified proteins. Proteins are enzymatically labeled utilizing the permissive mutant β-1,4-galactosyltransferase (Gal-T1 (Y289L)) which transfers azido-modified galactose (GalNAz) from UDP-GalNAz to O-GlcNAc residues. Then, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT™ Glycoprotein Detection kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne). Labeling and detection can be completed in less than 24 hours and the Click-iT™ glycoprotein products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of this important post-translational modification.

사양

그린 기능Less hazardous

제품 유형O-GlcNAc Enzymatic Labeling System

수량1 Kit

검출 방법Biotin-based, Fluorescence

배송 조건Wet Ice

제품라인Click-iT™

라벨 또는 염료Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, Biotin, Oregon Green 488, TMR (Tetramethylrhodamine)

Labeling TargetProteins

Unit SizeEach