
Thermo Fisher Scientific Click-IT O-GlcNAc Enzymatic Labeling System
고감도 O-GlcNAc 단백질 표지를 위한 효소 기반 시스템으로, 클릭 반응을 이용한 신속한 검출 가능. LC-MS/MS 및 Multiplexed Proteomics™ 분석 호환. 24시간 이내 라벨링 및 검출 완료. 다양한 형광 및 비오틴 라벨 옵션 제공.
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Invitrogen™ Click-IT™ O-GlcNAc Enzymatic Labeling System
The Click-iT™ O-GlcNAc Enzymatic Labeling System provides a highly sensitive and efficient method for the in-vitro modification of O-GlcNAc-modified proteins.
Proteins are enzymatically labeled utilizing the permissive mutant β-1,4-galactosyltransferase (Gal-T1 (Y289L)), which transfers azido-modified galactose (GalNAz) from UDP-GalNAz to O-GlcNAc residues.
Then, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can be detected using a Click-iT™ Glycoprotein Detection Kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne).
Labeling and detection can be completed in less than 24 hours, and the Click-iT™ glycoprotein products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of this important post-translational modification.
제품 사양
| 항목 | 내용 |
|---|---|
| 제품 유형 | O-GlcNAc Enzymatic Labeling System |
| 수량 | 1 Kit |
| 검출 방법 | Biotin-based, Fluorescence |
| 배송 조건 | Wet Ice |
| 제품 라인 | Click-iT™ |
| 라벨 또는 염료 | Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, Biotin, Oregon Green 488, TMR (Tetramethylrhodamine) |
| Labeling Target | Proteins |
| Unit Size | Each |
| 그린 기능 | Less hazardous |
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