Thermo Fisher Scientific EIF2S1 Monoclonal Antibody (OTI1H1), TrueMAB
EIF2S1 단백질을 인식하는 Mouse monoclonal 항체로 Western blot, IHC, Flow Cytometry에 적합. Human 시료 반응성. 동결건조 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H1 |
| Immunogen | Full length human recombinant protein of human EIF2S1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma).
This translation initiation factor mediates binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex.
The protein can be phosphorylated by stress-activated kinases, and phosphorylated EIF2A inhibits EIF2B activity, preventing guanine nucleotide exchange.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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