Thermo Fisher Scientific Acetyl-NFkB (Lys310) Polyclonal Antibody
NFkB p65의 Lys310 아세틸화를 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ELISA, IP에 사용 가능. 인간, 생쥐, 닭, 원숭이, 랫드 반응성. 친화 크로마토그래피로 정제된 액상 제품으로 -20°C 보관. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:50,000 |
| Immunoprecipitation (IP) | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-NFkB K310ac affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 310 of human TF65 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.05 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 50% glycerol, 1mg/mL BSA |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
A BLAST analysis suggests cross-reactivity with mouse and human based on 100% sequence homology.
Cross-reactivity with NFkB K310ac from other sources has not been determined.
Target Information
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is located on chromosome 11 in humans.
NF-kB p65, also known as RelA, belongs to the Rel family of proteins and forms heterodimers with p50 or p52 subunits of NF-kB.
Upon TNFα binding to its receptor, IKK is phosphorylated, leading to phosphorylation and degradation of IκB, which releases NF-kB to translocate into the nucleus.
NF-kB p65 plays a key role in transcription of immunoglobulin κ gene in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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