Thermo Fisher Scientific PrP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Frozen) (IHC (F))

1:20

-

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Sheep

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: G(90) Q G G G T H N Q W N K P(102) G G C if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PrP,uniProtId:P23907-1,ncbiNodeId:9940,antigenRange:90-102,antigenLength:256,antigenImageFileName:PA1-750_PrP_P23907-1_Rabbit.svg,antigenImageFileNamePDP:PA1-750_PrP_P23907-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2172277

Product Specific Information

PA1-750 detects prion protein (PrP) from sheep tissues.

PA1-750 has been successfully used in Western blot, immunoprecipitation and immunohistochemistry procedures. By Western blot, this antibody detects a 33-35 kDa protein representing PrPC and a 27-30 kDa protein representing PrPSc from sheep normal and infected brains. Immunohistochemical staining of PrP in formalin-fixed infected sheep brain with PA1-750 results in intense staining of PrP plaques which are known to exhibit an abnormally high concentration of PrP.

PA1-750 immunizing peptide corresponds to amino acid residues 90-102 from hamster PrP. PA1-750 immunizing peptide (Cat. # PEP-034) is available for use in neutralization and control experiments.

Target Information

Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.