Invitrogen™

PureLink™ 96 HQ Plasmid DNA Purification Kit

The PureLink HQ 96 Plasmid DNA Purification Kit isdesigned for high-throughput isolation of high-qualityplasmid DNA from bacterial cells. It can자세히 알아보기

The PureLink HQ 96 Plasmid DNA Purification Kit isdesigned for high-throughput isolation of high-qualityplasmid DNA from bacterial cells. It can beused with a vacuum manifold or a centrifuge and iscompatible with automated liquid handling workstations.

The PureLink HQ 96 Plasmid DNA Purification Kit offersthe following advantages:
• Ability to isolate up to 10 μg plasmid DNA per well
• Designed to isolate high-quality plasmid DNA in30-45 minutes
• Minimal genomic DNA contamination in the purifiedplasmid DNA
• Compatible with automated liquid handlingworkstations
• Reliable performance of the purified plasmid DNA indownstream applications such as mammaliantransfection

Process overview
Bacterial cells are harvested and resuspended in ResuspensionBuffer with RNase. The cells are lysed using an alkaline/SDScell lysis procedure. The lysate is then neutralized andconditions are adjusted for subsequent binding. After lysateclarification using the PureLink HQ 96 Clarification Plate, thelysate is processed through the PureLink HQ 96 BindingPlate. The DNA binds to the silica-based membrane in theplate and impurities are removed by washing with WashBuffer. The DNA is then eluted in low salt Elution Buffer orwater.

사양

형식96-well Plate

Isolation TechnologySilica Plate

샘플 종류Bacterial Culture

최종 제품 유형Plasmid DNA

용도(장비)Automated Liquid Handling Workstation

용도(애플리케이션)PCR, Transfection, Sequencing, Transformation, Cloning

고처리량 호환성High-throughput Compatible

반응 수4 x 96 Preps

프렙 스케일< 100 μg (Small-Scale) Plasmid DNA

제품라인PureLink™

제품 유형Plasmid DNA Purification Kit

수량4 x 96 preps

배송 조건Room Temperature

타겟Plasmid DNA

테스트 시간30 to 40 min.

Unit SizeEach