Gibco™

Expi293™ Inducible Expression System Kit

The Expi293 Inducible Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian자세히 알아보기

The Expi293 Inducible Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The Expi293F Inducible cell line was established by the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. This cell line is a powerful tool for tetracycline-inducible high-yield expression of recombinant proteins.

Expi293 Inducible Expression System Kit features include:
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

The components included in the Expi293 Inducible Expression System Kit are: one vial of frozen Expi293F Inducible cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one pcDNA5/TO Mammalian Expression Vector kit, 500 mg tetracycline hydrochloride, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 Inducible Expression System are also available for individual purchase.

사양

유형Inducible Expression System Kit

배달 유형Transfection

수량1 Kit

배송 조건Approved for shipment at Room Temperature or on Wet or Dry Ice

시작 물질DNA

세포주Expi293F™

제품라인Expi293™

용도(애플리케이션)Protein Expression

프로모터CMV/TO

Unit SizeEach