Thermo Fisher Scientific Expi293 Inducible Expression System Kit

Gibco™ Expi293™ Inducible Expression System Kit

The Expi293 Inducible Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells.
This system utilizes engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, enabling tetracycline-regulated protein expression.
The Expi293F Inducible cell line was established through stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter.
This cell line provides a powerful tool for tetracycline-inducible, high-yield recombinant protein expression.

주요 특징

• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells (up to 1 g/L)
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent with specialized enhancers

구성품

• 1 vial of frozen Expi293F Inducible cells
• 1 L Expi293 Expression Medium
• 1 ExpiFectamine 293 Transfection Kit (sufficient for 1 L culture)
• 100 mL Opti-Plex Complexation Buffer
• 1 pcDNA5/TO Mammalian Expression Vector Kit
• 500 mg Tetracycline Hydrochloride
• pRABBIT IgG IRES-EmGFP positive control vector

All components of the Expi293 Inducible Expression System are also available for individual purchase.

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