Thermo Fisher Scientific Rabbit anti-Sheep IgG (H+L) Secondary Antibody, HRP

Applications

Tested Dilution

Western Blot (WB)

1:2000-1:20,000

Immunohistochemistry (IHC)

1:500-1:5,000

Immunocytochemistry (ICC/IF)

1:500-1:5,000

Product Specifications

Species Reactivity

Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

HRP HRP HRP

View additional formats

• [AP](/antibody/secondary/product-name/Sheep IgG (H--L)/conjugates/AP/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Sheep IgG (H--L)/conjugates/Biotin/host/Rabbit/)
• [FITC](/antibody/secondary/product-name/Sheep IgG (H--L)/conjugates/FITC/host/Rabbit/)
• Request custom conjugation

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_228457

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

This antibody has been successfully used in Western blot, and ICC applications.

Antibody Specificity: The antibody reacts with the heavy chains of sheep IgG and with light chains common to most sheep immunoglobulins, based on immunoelectrophoresis. No antibody was detected against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Restoration and Storage: Store product at 4°C until opened. Restore with 1.5 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.

To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Country of Origin: USA

Target Information

Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.