Thermo Fisher Scientific KIT Monoclonal Antibody (UMAB216), UltraMAB
Human KIT 단백질(aa 27-308)을 항원으로 제작된 UMAB216 단클론 항체로, IHC(P)에서 1:400 희석으로 검증됨. 무보존제, 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. 연구용으로 KIT 관련 세포 성장 및 신호전달 분석에 적합.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1:400
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB216 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 27–308 of human KIT produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
KIT (c-KIT) is a proto-oncogene encoding a type III transmembrane receptor for mast cell growth factor (MGF, also known as stem cell factor, SCF).
KIT regulates growth and activation of various hematopoietic and non-hematopoietic cells. Mutations are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
Activation of KIT by SCF leads to receptor dimerization, autophosphorylation (including Tyr703), and downstream signaling via Ras/ERK, PI3K, PLC-γ, and JAK/STAT pathways.
KIT receptor activity is essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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