Thermo Fisher Scientific AKAP8 Polyclonal Antibody
AKAP8 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, 인간 및 랫트 시료에 반응합니다. Western blot, IHC, ELISA에 적합하며 고순도 친화 크로마토그래피 정제 제품입니다. 액상 형태로 제공되며 -20°C에서 보관합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 592–692 of human AKAP8 (NP_005849.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.49 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS (pH 7.3) with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901375 |
Product Specific Information
- Positive Samples: BT-474, HL-60, SKOV3, HeLa
- Immunogen Sequence:
EGAPAPESSG EPAEDEGPTD TAEAGSDPQA EQLLEEQVPC GTAHEKGVPK ARSEAAEAGN GAETMAAEAE SAQTRVAPAP AAADAEVEQT DAESKDAVPT E
Target Information
The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by compartmentalization of the kinase within the cell. The R subunit (RII) of PKA interacts with specific RII-anchoring proteins, collectively known as A-kinase anchoring proteins (AKAP).
AKAP95 (AKAP8) is a nuclear matrix protein expressed in liver, heart, pancreas, kidney, and skeletal muscle. During mitosis, AKAP95 is recruited to chromosomes and plays an essential role in mitotic progression. It participates in PKA signaling through interaction with the RII regulatory subunit and forms a complex with HA95 and HDAC3, required for deacetylation of Histone H3 in mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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