Thermo Fisher Scientific Methotrexate VHH-8His-Cys-tag Recombinant Alpaca Monoclonal Antibody (VHH4)

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Alpaca / VHH

Expression System

CHO cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

VHH4

Immunogen

MTX/Methotrexate-KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Metal-chelate chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Compatible with Direct ELISA

Target Information

Methotrexate is an antimetabolite and antifolate agent with antineoplastic and immunosuppressant activities. Methotrexate binds to and inhibits the enzyme dihydrofolate reductase, resulting in inhibition of purine nucleotide and thymidylate synthesis and, subsequently, inhibition of DNA and RNA syntheses. Methotrexate also exhibits potent immunosuppressant activity although the mechanism(s) of actions is unclear. [National Center for Biotechnology Information. PubChem Compound Database; CID=126941].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.