Thermo Fisher Scientific beta Adaptin/AP2 beta Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 polyclonal rabbit antibody로, beta Adaptin/AP2 beta 단백질 검출에 사용됩니다. IHC, ICC/IF, ELISA 등 다양한 응용에 적합하며, Protein A로 정제된 1 mg/mL 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human beta Adaptin, amino acids 351–450 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin-coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors, which form a layer of protein coat between the clathrin lattice and the membrane.
There are two characterized adaptors, AP1 and AP2. AP1 is associated with clathrin-coated vesicles at the trans-Golgi network, and AP2 is associated with endocytic clathrin-coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor.
AP2 is composed of four subunits: two separate ~100 kDa gene products with similar domain structures (alpha and beta adaptin), and ~50 and ~17 kDa subunits. There are two alpha-adaptin genes, alpha A and alpha C, which have a tissue-specific pattern of expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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