Thermo Fisher Scientific STRO-1 Monoclonal Antibody (STRO-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 9 publications 9 publications

Flow Cytometry (Flow)

Assay-dependent

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

STRO-1

Immunogen

CD34+ human bone marrow cells

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2533437

Target Information

STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow. STRO-1 binds to approximately 10% of bone marrow mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage (CFU-GM), erythroid bursts (BFU-E), and mixed colonies (CFU-Mix). From bone marrow cells, the frequency of fibroblast colony forming cells (CFU-F) is enriched approximately 100-fold in the STRO-1-positive/Glycophorin A-negative population than in the STRO-1-positive/Glycophorin A-positive population. When plated under long-term BM culture conditions, STRO-1-positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. A STRO-1-positive enriched subset of marrow cells is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts and chondrocytes. STRO-1 is a valuable antibody for the identification, isolation and functional characterization of human bone marrow stromal cell precursors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.