Thermo Fisher Scientific POLI Monoclonal Antibody (8G9)
POLI 단백질을 표적으로 하는 Thermo Fisher Scientific의 마우스 단클론 항체(8G9). Western blot과 ELISA에 적합하며 인간 시료에 반응. 액상 형태로 제공되며 보존제가 포함되지 않음. 연구용으로만 사용 가능.
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Thermo Fisher Scientific POLI Monoclonal Antibody (8G9)
Applications and Tested Dilution
- Western Blot (WB): 1:500–1:1,000
- ELISA: 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG3, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8G9 |
| Immunogen | POLI (AAH32662, 616–715 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Storage Buffer | Ascites |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
PQGFHFTNSN PAVSAFHSFP NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE KITFPSDIDP QVFYELPEAV QKELLAEWKR AGSDFHIGHK
Target Information
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it preferentially inserts guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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