Thermo Fisher Scientific PAAF1 Monoclonal Antibody (OTI2G6), TrueMAB
Human PAAF1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot에 최적화됨. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능. Proteasome 26S 조립 억제 관련 연구에 적합. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G6 |
| Immunogen | Full length human recombinant protein of human PAAF1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
PAAF1 inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core.
In case of HIV-1 infection, PAAF1 is recruited by viral Tat to the HIV-1 promoter, promoting 19S regulatory complex recruitment through dissociation of the proteasome 26S.
This process enhances provirus transcription efficiency.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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