Thermo Fisher Scientific UNG Monoclonal Antibody (OTI1G2), TrueMAB
Thermo Fisher의 UNG 단클론 항체(OTI1G2)는 인간 UNG 단백질을 인식하는 Mouse IgG2b 항체입니다. Western blot 및 IHC(P)에서 검증되었으며, 액상 형태로 제공됩니다. 세포 내 uracil-DNA glycosylase 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G2 |
| Immunogen | Full length human recombinant protein of human UNG produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB.
The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively), and the corresponding mRNAs are not resolved as two species in gel electrophoresis.
Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity.
A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues.
The level and expression pattern of UNG1 and UNG2 differ between cell and tissue types. Additionally, the expression of UNG is cell cycle regulated, being generally higher in proliferating than in nonproliferating tissues and cells.
UNG1 is a 304 amino acid protein, and UNG2 is a 313 amino acid protein.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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