Thermo Fisher Scientific alpha Adducin Polyclonal Antibody
Rabbit polyclonal antibody against alpha Adducin for WB and ELISA applications. Recognizes human and mouse proteins. Affinity purified, liquid form, unconjugated, stored at -20°C. Suitable for cytoskeleton and membrane protein research.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
ELISA
- Tested Concentration: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 60–300 of human ADD1 (NP_0549082) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.78 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806248 |
Product Specific Information
Immunogen sequence:
MILQSPAFCE ELESMIQEQF KKGKNPTGLL ALQQIADFMT TNVPNVYPAA PQGGMAALNM SLGMVTPVND LRGSDSIAYD KGEKLLRCKL AAFYRLADLF GWSQLIYNHI TTRVNSEQEH FLIVPFGLLY SEVTASSLVK INLQGDIVDR GSTNLGVNQA GFTLHSAIYA ARPDVKCVVH IHTPAGAAVS AMKCGLLPIS PEALSLGEVA YHDYHGILVD EEEKVLIQKN LGPKSKVLIL R
Positive Samples: BT-474, Mouse brain
Cellular Location: Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, Cytoskeleton
Target Information
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein composed of related subunits produced from distinct genes but sharing similar structure.
- Alpha- and beta-adducin include a protease-resistant N-terminal region and a hydrophilic, protease-sensitive C-terminal region.
- Alpha- and gamma-adducins are ubiquitously expressed.
- Beta-adducin is highly expressed in brain and hematopoietic tissues.
- Adducin binds with high affinity to Ca(2+)/calmodulin and serves as a substrate for protein kinases A and C.
- Alternative splicing produces multiple isoforms, though not all have been fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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