Applied Biosystems™

7900HT Fast Real-Time PCR System with 384-Well Block Module

The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and자세히 알아보기

The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan™ Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability.

• Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate
• Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction
• Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences
• 96- or 384-well plate compatibility (including the TaqMan™ Low Density Array)
• Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security
• Hands-free plate-loading and unloading provides true walkaway automation allowing you to increase your lab`s productivity
• Proven assay development guidelines save time and money

High Throughput
The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. An optional Automation Accessory (not included) combined with 384-well plate capability make the 7900HT system ideally suited to meet the high-throughput requirements of todays drug discovery process. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5 nuclease assay.

Flexibility
The 7900HT system is a versatile research tool that can accommodate any real-time PCR need. User-interchangeable thermal cycling block formats let you select the format that`s right for your project, using industry-standard 96- and 384-well formats, as well as a novel 384-well TaqMan™ Low Density Array and a new Fast 96-well block that reduces run times from 2 hours to about 30 minutes. An easy automation upgrade path lets you add features to meet throughput demands. A Fast PCR option reduces run times from 2 hours to about 30 minutes.

Powerful Software
Using two automation software tools, Plate Utility and Automation Controller, most of the gene expression and SNP genotyping workflow is fully automated for high-throughput applications and requires minimal user intervention. You can now process 5,000 x 384 well plates (384 markers), which equates to 1.92 million genotypes. Using Relative Quantification (RQ) Manager, you can also process 200 x 384-well plates (96 detectors with quadruplicate data points⁄multiplexed endogenous control), which equates to 153,600 data points. Fully integrated into one complete Enterprise system, SNP Manager and RQ Manager eliminate data analysis bottlenecks in high-throughput SNP and gene expression research by allowing significantly more plates to be analyzed simultaneously. This reduces hands-on analysis time and simplifies the data analysis workflow.

For Research Use Only. Not for use in diagnostics procedures.

사양

블록 형식Interchangeable

형식384-well Plate

열 정확도±0.25°C of Display Temperature

샘플 램프 속도Fast Mode: ± 2.2°C⁄s, Standard mode: ± 1.6°C⁄s

민감도Down to 10 Copies

온도 범위(미터법)4 to 100°C

반응 부피 범위5–20 μL

패시브 참조 염료ROX (Separate Tube), No ROX, Other Passive Reference Dye, ROX (Pre-mixed)

동적 범위Up to 9 Logs of Linear Dynamic Range

보정된 염료SYBR™ Green I, ROX™, NED™, JOE™, TAMRA™, VIC™, FAM™, TET™

고처리량 호환성High-throughput Compatible, Multiplexing

용도(장비)7900HT Fast System, AB 7900 HT 384-Well System

열 균일성±0.5°C

반응 속도Fast

처리량≥5,000 Real Time PCR rxns per day (384-well block and automation accessory), ≥10,000 endpoint PCR rxns per hour (384-well block and automation accessory)

검출 방법SYBR, Primer-Probe Detection

실행 시간52 min. (384-well in Fast Mode), <2 hrs (384-well in Standard Mode)

옵틱스Extended-life 488 nm Argon-ion Laser Excitation Source, No Filters (CCD acts as spectrograph with continuous detection 500 to 650 nm)

열 사이클링 시스템Peltier-based System

치수72 cm (W) x 84 cm (D) x 64 cm (H) (with Drawer Open)

용량Up to 84 x 384-well Plates with Optional Automation Accessory

수량1 instrument

무게114 kg with Automation Accessory, 82 kg without Automation Accessory

Unit SizeEach