Thermo Fisher Scientific HNMT Polyclonal Antibody
Thermo Fisher Scientific의 HNMT Polyclonal Antibody는 인간 및 생쥐 시료에 반응하며 Western blot, ELISA, IP에 적합합니다. 재조합 HNMT(aa 1-292)를 면역원으로 사용한 rabbit polyclonal 항체로, PBS/glycerol buffer에 보관되며 -20°C에서 안정적입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–292 of human HNMT (NP_008826.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805456 |
Product Specific Information
Immunogen sequence:
MASSMRSLFS DHGKYVESFR RFLNHSTEHQ CMQEFMDKKL PGIIGRIGDT KSEIKILSIG GGAGEIDLQI LSKVQAQYPG VCINNEVVEP SAEQIAKYKE LVAKTSNLEN VKFAWHKETS SEYQSRMLEK KELQKWDFIH MIQMLYYVKD IPATLKFFHS LLGTNAKMLI IVVSGSSGWD KLWKKYGSRF PQDDLCQYIT SDDLTQMLDN LGLKYECYDL LSTMDISDCF IDGNENGDLL WDFLTETCNF NATAPPDLRA ELGKDLQEPE FSAKKEGKVL FNNTLSFIVI EA
Positive Samples: HT-29, HepG2, HeLa, Mouse liver, Mouse kidney, Mouse brain
Cellular Location: Cytoplasm
Target Information
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase (HNMT) and oxidative deamination via diamine oxidase.
HNMT is a cytosolic enzyme that uses S-adenosyl-L-methionine as a methyl donor. In the mammalian brain, histamine neurotransmitter activity is regulated by N(tau)-methylation because diamine oxidase is absent in the central nervous system.
A common genetic polymorphism affects the activity levels of this enzyme in red blood cells.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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