Thermo Fisher Scientific NAPEPLD Monoclonal Antibody (OTI5F7), TrueMAB
NAPEPLD 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC, Flow cytometry에 적합. Human 시료 반응성. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5F7 |
| Immunogen | Full length human recombinant protein of human NAPEPLD produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a membrane-bound enzyme that releases the endocannabinoid anandamide and other bioactive N-acylethanolamines from NAPEs in animal tissues.
NAPE-PLD is expressed in specific neuronal populations and localized to axonal processes.
NAEs generated by NAPE-PLD may act as synaptic signaling molecules regulating postsynaptic neuron activity.
It also catalyzes the hydrolysis of NAPE to generate OEA (oleoylethanolamide), a lipid amide that inhibits food intake in rodents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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