
Thermo Fisher Scientific Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, DyLight 680
DyLight 680 형광 표지를 가진 염소 유래 항-인간 IgG Fc 2차 항체로, 다양한 면역실험(WB, IHC, ICC, Flow)에 사용 가능. 교차 흡착 처리로 비특이적 반응 최소화. 높은 순도와 안정성으로 신뢰성 있는 형광 검출 제공.
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Thermo Fisher Scientific Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, DyLight 680
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgG-Fc Fragment |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556718 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, chicken, horse, mouse, rabbit, rat, and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, horse, mouse, rabbit, rat, and sheep.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies enable versatile detection systems (HRP, AP, fluorescence) and provide enhanced sensitivity through signal amplification.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
- AP
- Biotin
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 755
- DyLight 800
- FITC
- HRP
- TRITC
- Request custom conjugation
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