Invitrogen™

pBAD202 Directional TOPO™ Expression Kit

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of자세히 알아보기

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein.

In addition to being a more effective way to clone, TOPO™ Cloning eliminates these potential expression problems. TOPO™ Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).

Many of Invitrogen`s powerful expression vectors are available adapted for one-step TOPO™ cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO™ Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.

사양

구성 또는 유도성 시스템Inducible

유도제Arabinose

프로모터araBAD

제품 유형TOPO Expression Kit

선택 제제(진핵)None

항생제 내성 박테리아Kanamycin (KanR)

박테리아 또는 효모 균주LMG194, TOP10

분열EK (Enterokinase) Recognition Site

발현 시스템E. coli

발현 메커니즘Cell-Based Expression

단백질 태그His Tag (6x), V5 Epitope Tag, Thioredoxin

클로닝 방법Directional TOPO

수량20 reactions

벡터pBAD

제품라인TOPO™

Unit SizeEach