Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), FITC, eBioscience
FITC로 결합된 IM7 클론의 CD44 단일클론 항체로, 마우스 및 인간 시료에 반응합니다. Flow cytometry에 최적화되어 있으며 0.5 µg/test로 사용 가능합니다. 488 nm에서 여기되고 520 nm에서 방출됩니다. 세포 접착 및 이동 연구에 적합합니다.
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- 11-0441-8x (4개 옵션)
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Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 4 publications |
| Immunohistochemistry (IHC) | - | View 16 publications |
| Immunocytochemistry (ICC/IF) | - | View 17 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 301 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Fruit fly, Gerbil, Horse, Human, Mouse, Non-human primate, Pig, Rat |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IM7 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465044 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV / Violet / Yellow / Red / Blue series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright series
- NovaFluor series
- Functional Grade
- Custom conjugation available
Product Specific Information
The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen, and peripheral B and T cells can upregulate CD44 expression. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse bone marrow cells and splenocytes at ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD44 is a 100 kDa type 1 transmembrane glycoprotein expressed on human leukocytes, brain white matter, and epithelial cells of the intestine and breast. It serves as a receptor for hyaluronic acid (HA) and mediates cell-cell interactions, adhesion, and migration. CD44 plays roles in lymphocyte activation, recirculation, and homing. Expression may increase in certain carcinomas, potentially related to metastasis.
CD44 is expressed by hematopoietic and non-hematopoietic cells, epithelial tissues, and filopodia in cultured keratinocytes. Bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate its expression upon stimulation. The CD44 gene undergoes complex alternative splicing, generating diverse isoforms. Disorders associated with CD44 dysfunction include superficial keratitis and lichen sclerosus.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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