Thermo Fisher Scientific CPSF160 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predicted to react with Bovine

Target Information

Midline-1 (Tripartite motif-containing protein 18, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1.
Midline-1 belongs to the TRIM/RBCC family and contains:

• Two B box-type zinc fingers
• One B30.2/SPRY domain
• One COS domain
• One fibronectin type-III domain
• One RING-type zinc finger

Midline-1 is believed to have E3 ubiquitin ligase activity, targeting the catalytic subunit of protein phosphatase 2 for degradation.
It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2 and interacts with IGBP1 (Lymphocyte signaling protein A4).

Defects in MID1 cause Opitz syndrome type I (OS-I), an X-linked recessive disorder characterized by:

• Hypertelorism
• Genital-urinary defects (e.g., hypospadias in males, splayed labia in females)
• Lip-palate-laryngotracheal clefts
• Imperforate anus
• Developmental delay
• Congenital heart defects

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.