Thermo Fisher Scientific C1orf55 Polyclonal Antibody
C1orf55 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태이며, 4°C에서 보관. DNA 복제 및 세포 주기 조절 연구에 적합.
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Thermo Fisher Scientific C1orf55 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 350 and 400 of human chromosome 1 open reading frame 55 (SDE2, UniProt ID: Q6IQ49-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
C1orf55 (SDE2) is involved in DNA replication and cell cycle control. Unprocessed SDE2 interacts with PCNA via its PIP-box, preventing monoubiquitination of PCNA and inhibiting translesion DNA synthesis. The interaction leads to processing by a deubiquitinating enzyme, removing the N-terminal ubiquitin-like domain. Mature SDE2 is degraded by the DCX(DTL) complex in a cell cycle- and DNA damage-dependent manner. Binding of SDE2 to PCNA counteracts UV-induced replication stress, and complete degradation of SDE2 is necessary for S-phase progression. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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