Thermo Fisher Scientific ATG16L1 Monoclonal Antibody (54CT27.2.6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:60

-

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

54CT27.2.6

Immunogen

purified His-tagged ATG16L1 protein (fragment)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897402

Target Information

Autophagy related protein 16 L is encoded by the ATG16L gene and is located on chromosome 2 in humans. It is also known as WD30 or IBD10 (Inflammatory Bowel Disease 10). ATG 12 and ATG5 conjugate with each other and undergoes homo oligomerisation with ATG16L to form an 800 kDa complex called the Atg16L complex. This complex facilitates coupling or lipidation of LC3 to phosphatidylethanolamine (PE) and this ubiquitin-like conjugation system is a widely used marker for autophagy. However, the ATG 16L complex along with other ATGs like ATG 18 and ATG 21 prevent the premature cleavage of ATG8-PE by the cysteine protease, Atg4 until the autophagosome is deemed complete.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.