Invitrogen™

MAX Efficiency™ DH5α Competent Cells

MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by자세히 알아보기

MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors. In addition to supporting blue/white screening, _rec_A1 and _end_A1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Benefits of MAX Efficiency DH5α Competent Cells:
• Versatile strain for everyday cloning applications
• High quality plasmid preparations
• Useful for generating plasmid libraries

DH5α c**ompetent** c**ells** for high transformation efficiency
The Φ80_lac_ZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α cells are capable of being transformed efficiently with large plasmids, and can also serve as a host for the M13mp cloning vectors if a lawn of DH5αF′IQ (or similar) is provided to allow plaque formation.

Features of MAX Efficiency DH5α Competent Cells include:
• Transformation efficiency of >1 x 109 transformants/μg plasmid DNA
• High plasmid yield from the DH5α (end_A1) _E. coli strain
• Blue/white screening capable (_lac_ZΔM15)
• Greater insert stability (_rec_A1)

Genotype:
F- Φ80_lac_ZΔM15 Δ(_lac_ZYA-_arg_F) U169 _rec_A1 _end_A1 _hsd_R17 (rk-, mk+) _pho_A _sup_E44 λ-_thi_-1 _gyr_A96 _rel_A1

Find the strain and format that fits you needs
• DH5α strain and its derivatives available in with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats
• ElectroMAX™ DH5α-E Competent Cells are engineered for electroporation to achieve high transformation efficiency of >1 x 1010
• DH5α T1R competent cells are available in MultiShot format for high throughput applications

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝No

형질전환 효율 수준High Efficiency (>1 x 109 cfu/μg)

항생제 내성 박테리아No

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

형식Tube

제품라인MAX Efficiency™

수량5 x 200 μL

Unit SizeEach