Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (P-Tyr-01), Alexa Fluor 647
Alexa Fluor 647로 표지된 phosphotyrosine 단일클론 항체로, 활성화된 세포 내 tyrosine phosphorylation 검출에 적합. Western blot, ICC/IF, Flow cytometry 등 다양한 응용 가능. 4°C 암소 보관, size-exclusion chromatography로 정제된 고품질 항체.
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Applications
Western Blot (WB)
- Assay-dependent
Immunocytochemistry (ICC/IF)
- Assay-dependent
Flow Cytometry (Flow)
- 1–5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | P-Tyr-01 |
| Immunogen | Phosphotyrosine conjugated to bovine serum albumin |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539546 |
Additional Formats
Product Specific Information
This antibody detects tyrosine phosphorylation in activated cells.
Target Information
Tyrosine phosphorylation plays a critical role in signal transduction from transmembrane receptors and oncogene tyrosine kinases. It is a key mechanism in mitogenesis, cell cycle progression, and oncogenic transformation, and continues to be actively studied in differentiation and intercellular communication.
Antibodies recognizing phosphorylated tyrosine residues are essential tools for studying tyrosine-phosphorylated proteins and their biochemical pathways.
The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for detecting P-Tyr proteins in immunohistochemical and immunocytochemical assays where secondary antibody use may complicate detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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