Thermo Fisher Scientific EEF1G Recombinant Rabbit Monoclonal Antibody (JE54-03)
EEF1G 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. 인간, 생쥐, 랫트 시료에 반응하며, HEK293 세포에서 발현된 재조합 항체. 단백질 A로 정제되어 높은 특이성과 재현성을 제공.
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- MA536168
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE54-03 |
| Immunogen | Recombinant protein within Human Elongation factor 1-gamma (aa 1–163) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2890440 |
Target Information
EF-1 (elongation factor-1) is a multi-protein complex responsible for the delivery of aminoacyl-tRNAs to the ribosome. EF-1 gamma (EEF1G or GIG35) is a 437 amino acid subunit of the EF-1 complex. It is expressed in stomach, pancreas, brain, lung, kidney, intestine, liver, and spleen. EF-1 gamma contains an N-terminal Glutathione transferase domain thought to anchor the complex to cellular components and may play a role in assembling multiprotein complexes containing aminoacyl-tRNA synthetases. Increased expression of EF-1 gamma is associated with pancreatic cancer, suggesting its involvement in oncogenic transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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