Thermo Fisher Scientific CD10 (Membrane Metalloendopeptidase) Monoclonal Antibody (MME, 1893)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• For formalin-fixed tissues, heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.

Target Information

CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA) is a cell surface enzyme with neutral metalloendopeptidase activity that inactivates various biologically active peptides.
It is expressed on lymphoblastic, Burkitt’s, and follicular germinal center lymphoma cells, immature B cells in adult bone marrow, and cells from patients with chronic myelocytic leukemia (CML).
CD10 is also found on breast myoepithelial cells, bile canaliculi, fibroblasts, and highly expressed on kidney and gut epithelial brush borders.
As a neutral endopeptidase, CD10 cleaves peptides at the amino side of hydrophobic residues and inactivates peptide hormones such as glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
It is a 100 kDa type II transmembrane glycoprotein encoded by a >45 kb single-copy gene. The 5′ untranslated region is alternatively spliced, producing four mRNA transcripts without affecting the coding region.
Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie-Tooth disease.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.