Thermo Fisher Scientific BCR Monoclonal Antibody (2E5)
Thermo Fisher Scientific의 BCR Monoclonal Antibody (Clone 2E5)는 인간 BCR 단백질을 인식하는 Mouse IgG2a 항체로, WB, ICC/IF, ELISA, PLA 등 다양한 분석에 사용 가능. 고순도 친화 크로마토그래피 정제, PBS 버퍼에 보존, 연구용으로만 사용.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.1 ng/mL |
| in situ PLA (PLA) | 1:1200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E5 |
| Immunogen | BCR (NP_004318.3, 182–280 a.a) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
FHHERGLVKV NDKEVSDRIS SLGSQAMQME RKKSQHGAGS SVGDASRPPY RGRSSESSCG VDGDYEDAEL NPRFLKDNLI DANGGSRPPW PPLEYQPYQ
Target Information
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein encoded by sequences from both BCR and ABL (chromosome 9). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product remains unclear. The protein has serine/threonine kinase activity and acts as a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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