Thermo Scientific™

Pierce™ NEM (N-ethylmaleimide)

Thermo Scientific Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines),자세히 알아보기

Thermo Scientific Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.

Features of N-Ethylmaleimide (NEM):

• Permanently block sulfhydryls to prevent disulfide bond formation.
• Monitor the reaction by measuring the decrease in absorbance at 300nm
• Block sulfhydryl-containing reagents that interfere in enzyme assays

N-Ethylmaleimide (NEM) is an alkylating reagent that reacts with sulfhydryls. At pH 6.5-7.5, the maleimide reaction is specific for sulfhdryls; however, at pH values greater than 7.5, reactivity with amino groups occurs. Maleimide groups react with sulfhydryls by nucleophilic attack of the thiolate anion on one of the carbons of the double bond. When sufficient sulfhydryls have been blocked, the reaction can be monitored by measuring the decrease in absorbance at 300nm as the double bond becomes a single bond. The resulting thioether group is non-reversible and terminates in an ethyl group, blocking or capping the sulfhydryl.

Related Products
Pierce™ MMTS (methyl methanethiosulfonate)

사양

화학물질 반응성Thiol

라벨 또는 염료Unreactive Group

라벨 유형Blocking Groups

제품라인Pierce™

반응성 부분Maleimide

스페이서Medium (10 to 30 Å)

형태Solid

수량25 g

용해도Water

제품 유형NEM

Unit SizeEach