Thermo Fisher Scientific SIN3A Recombinant Rabbit Monoclonal Antibody (8V5B6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8V5B6

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1174-1273 of human mSin3A (Q96ST3) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SIN3A,uniProtId:Q96ST3-1,ncbiNodeId:9606,antigenRange:1174-1273,antigenLength:1273,antigenImageFileName:MA5-42782_SIN3A_Q96ST3-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42782_SIN3A_Q96ST3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.2 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911923

Product Specific Information

Positive test controls include: Mouse testis, Rat testis.

Immunogen sequence: YKMVYVIKSE DYMYRRTALL RAHQSHERVS KRLHQRFQAW VDKWTKEHVP REMAAETSKW LMGEGLEGLV PCTTTCDTET LHFVSINKYR VKYGTVFKAP

Target Information

Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.