Invitrogen™

One Shot™ TOP10/P3 Chemically Competent E. coli

One Shot TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic supF gene (tyrosine tRNA자세히 알아보기

One Shot TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic _sup_F gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other _sup_F-containing vector preparation.

One Shot TOP10/P3 strain features
• P3 plasmid for selection of sup_F-containing plasmids
• Mutated _end_A1 gene for increased quality of plasmid DNA preparations
• _recA1 for reduced occurrence of non-specific recombination in cloned DNA
• lacZΔM15 for blue/white color screening of recombinant clones

P3 Plasmid
The P3 plasmid is a low-copy-number, 60 kb plasmid that carries kanamycin, tetracycline and ampicillin resistance markers. The kanamycin gene is fully active and is used to select for cells carrying P3. Tetracycline and ampicillin genes carry amber mutations which render the genes inactive during normal growth and replication of the bacteria. Upon transformation of a vector carrying the suppressor F gene, the amber mutations in the tetracycline and ampicillin genes on the P3 plasmid are suppressed and the E. coli are resistant to these antibiotics.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG P3: KanR AmpR (am) TetR (am)

Find the strain and format that you need
TOP10 is offered in chemically competent and electrocompetent cell formats.
Choose MultiShot competent cells for high-throughput cloning applications.
Email us for more information about custom formats.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝Yes (mcrA)

형질전환 효율 수준Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)

항생제 내성 박테리아Yes (Streptomycin, Kanamycin, Ampicillin, Tetracycline)

불안정 DNA 클로닝No

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

형식Tube

제품라인One Shot™

수량21 x 50 μL

Unit SizeEach