Thermo Fisher Scientific One Shot TOP10/P3 Chemically Competent E. coli

Thermo Fisher Scientific One Shot™ TOP10/P3 Chemically Competent E. coli

Invitrogen™ One Shot™ TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic supF gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other supF-containing vector preparation.

One Shot TOP10/P3 Strain Features

• P3 plasmid for selection of supF-containing plasmids
• Mutated endA1 gene for increased quality of plasmid DNA preparations
• recA1 mutation for reduced occurrence of non-specific recombination
• lacZΔM15 for blue/white color screening of recombinant clones

P3 Plasmid

The P3 plasmid is a low-copy-number, 60 kb plasmid that carries kanamycin, tetracycline, and ampicillin resistance markers.

• The kanamycin gene is fully active and used to select cells carrying P3.
• Tetracycline and ampicillin genes carry amber mutations, inactive under normal growth.
• When transformed with a vector containing the supF suppressor gene, amber mutations are suppressed, conferring resistance to tetracycline and ampicillin.

Genotype

F⁻ mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG P3: KanR AmpR (am) TetR (am)

Available Formats

TOP10 is offered in both:

• Chemically competent
• Electrocompetent cell formats

For high-throughput cloning, choose MultiShot competent cells.
For custom formats, contact customorders@thermofisher.com.

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