Thermo Fisher Scientific Bim Polyclonal Antibody
Bim 단백질을 인식하는 Rabbit Polyclonal 항체로, Western Blot 및 Immunocytochemistry에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며, 2–8°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 25 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to 15 amino acids near the amino terminus of human Bim |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at –20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2551924 |
Product Specific Information
- A suggested positive control is K562 cell lysate.
- PA5-34572 can be used with blocking peptide PEP-1615.
Target Information
BIM belongs to the BCL-2 protein family. Members of this family form hetero- or homodimers and act as anti- or pro-apoptotic regulators involved in various cellular activities.
The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3) and interacts with other BCL-2 family proteins such as BCL2, BCL2L1/BCL-X(L), and MCL1, functioning as an apoptotic activator.
Expression can be induced by nerve growth factor (NGF) and the forkhead transcription factor FKHR-L1, suggesting a role in neuronal and lymphocyte apoptosis.
Transgenic mouse studies indicate that this gene is an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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