Thermo Fisher Scientific LAG3 Monoclonal Antibody (LG.F9)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

2-10 µg/mL

-

ELISA (ELISA)

0.01-0.1 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

LG.F9

Immunogen

Recombinant human LAG3-Fc (R&D, 2319-L3)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

LAG-3 (Lymphocyte activation gene-3), designated CD223, is a 70 kDa type I transmembrane protein that is a member of the immunoglobulin superfamily (IgSF). LAG-3 is expressed on activated CD4+ and CD8+ T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells. LAG-3 on activated CD4+CD25+ Treg cells plays a role in their suppressive activity. LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli. In mice, deletion of LAG-3 and another negative regulator, PD-1, facilitates anti-cancer response but also blocks self-tolerance and increases susceptibility to autoimmune diseases. In humans, antibody-mediated down-regulation of LAG-3 and PD-1 allows more effective control of chronic malaria, while in NOD (non-obese diabetic) mice, deletion of LAG-3 alone accelerates diabetes.GenScript Human LAG3 Antibody (LG.F9), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Recombinant human LAG3-Fc (R&D, 2319-L3).

Target Information

LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.