Gibco™

RPMI, no methionine

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a자세히 알아보기

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using our media selector tool.

This RPMI medium is modified as follows:

With	Without
• L-glutamine	• Methionine
• Phenol red	• HEPES

Gibco™ RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA, which are not found in Eagles Minimal Essential Medium or Dulbeccos Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% fetal bovine serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

사양

아미노산No Methionine

세포주HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas

세포 유형Leukemic Cells

분류Animal Origin-free

농도1 X

형태Liquid

제품 유형RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)

Serum LevelStandard Serum Supplementation

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

첨가제 포함Glutamine, Phenol Red, Sodium Bicarbonate

첨가제 없음No HEPES, No Sodium Pyruvate

용도(애플리케이션)Mammalian Cell Culture

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량500 mL

규제 상태RUO

배송 조건Room Temperature

Unit SizeEach