Invitrogen™

pTrcHis TOPO™ TA Expression Kit

The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for자세히 알아보기

The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from the trc promoter. The vector in each kit, pTrcHis-TOPO™ or pTrcHis2-TOPO™, includes a minicistron element for enhanced translation efficiency of eukaryotic proteins in E. coli. Both vectors are provided linearized and activated with topoisomerase I for 5-minute TOPO™ Cloning with >85% cloning efficiency. To simplify protein purification and detection, the pTrcHis-TOPO™ and pTrcHis2-TOPO™ vectors include the following features:

**pTrcHis-TOPO™

•** N-terminal Xpress™ epitope for detection with an Anti-Xpress™ Antibody
• N-terminal polyhistidine (6xHis) tag for purification using nickelchelating resin and detection with an Anti-HisG Antibody
• Enterokinase cleavage site for efficient removal of N-terminal fusion tags

pTrcHis2-TOPO™
• C-terminal c-myc epitope for detection with an Anti-myc Antibody
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) Antibody

사양

구성 또는 유도성 시스템Inducible

유도제IPTG

프로모터Trc, lacO

제품 유형TA Expression Kit

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR)

박테리아 또는 효모 균주TOP10

분열EK (Enterokinase) Recognition Site

발현 시스템E. coli

발현 메커니즘Cell-Based Expression

단백질 태그His Tag (6x), Xpress Epitope Tag

클로닝 방법TOPO-TA

수량20 reactions

벡터pTrc

제품라인TOPO™

Unit SizeEach