Thermo Fisher Scientific HIF3A Monoclonal Antibody (OTI2D2)
HIF3A 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot 및 IHC(P)에서 검증됨. 인간 시료 반응성, Mouse IgG1 아이소타입. 고순도 친화 크로마토그래피 정제, 액상 형태로 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 355-669 of HIF3A produced in E.coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724448 |
Target Information
Cell growth and viability are compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1 alpha, HIF-1 beta (Arnt 1), EPAS-1 (HIF-2 alpha), and HIF-3 alpha, induce glycolysis, erythropoiesis, and angiogenesis to restore oxygen homeostasis. These factors belong to the Per-Arnt-Sim (PAS) domain transcription factor family. Under hypoxic conditions, HIF-1 alpha is upregulated and forms a heterodimer with Arnt 1 to create the HIF-1 complex, which binds to the hypoxia responsive element (HRE) of target genes to activate transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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