Invitrogen™

SuperScript™ Direct cDNA Labeling Module, no purification

The SuperScript™ Direct cDNA Labeling System is a microarray labeling kit that combines the performance of SuperScript™ III Reverse Transcriptase자세히 알아보기

The SuperScript™ Direct cDNA Labeling System is a microarray labeling kit that combines the performance of SuperScript™ III Reverse Transcriptase (RT) with the simplicity of direct labeling to yield highly fluorescent labeled cDNA that accurately represents your initial mRNA sample. In this method (Figure 1), anchored oligo(dT) anneals to the mRNA template. SuperScript™ III RT extends from the priming site, incorporating fluorescent dNTPs directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using purification columns before hybridization to a microarray.

The SuperScript™ Direct cDNA Labeling System uses 800 units of SuperScript™ III RT to generate:

• High cDNA yields without an additional enzyme “spike” (Figure 2)
• Strong signal intensities (Figure 3)
• A fast and simple protocol that minimizes “hands on” time and provides superior reproducibility

사양

제품 유형cDNA Labeling Module

검출 방법Fluorescence

최종 제품 유형cDNA (Labeled)

라벨 또는 염료Alexa Fluor™ 555, Alexa Fluor™ 647, Cy™3, Cy™5

라벨 또는 염료 포함No

라벨링 타겟cDNA

라벨링 방법Direct Labeling

샘플 종류RNA

제품라인SuperScript™

수량30 Reactions

Unit SizeEach