Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), PerCP-eFluor 710, eBioscience
인간 CD11c를 인식하는 모노클로날 항체로, PerCP-eFluor 710 형광체가 결합되어 있습니다. Flow cytometry 분석에 최적화되어 있으며, 488 nm 레이저로 여기되어 710 nm에서 방출됩니다. 단핵구, 대식세포, NK 및 수지상세포 분석에 적합합니다.
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Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), PerCP-eFluor 710, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (1 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3.9 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10596368 |
Additional Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- eFluor 506
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation available
Product Specific Information
Description:
The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alphaX chain. CD11c associates with beta2 integrin to form the CD11c/CD18 heterodimer, expressed on monocytes, granulocytes, macrophages, NK cells, dendritic cells, and subsets of T and B lymphocytes. This complex binds CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
Applications Reported:
Suitable for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested on normal human peripheral blood cells. Recommended use: 5 µL (1 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited by a 488 nm blue laser. Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Ensure instrument compatibility.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal brightness loss.
Excitation / Emission / Laser:
488 nm / 710 nm / Blue Laser
Filtration:
0.2 µm post-manufacturing filtered
Target Information
CD11, with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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