Thermo Fisher Scientific LIN28A Monoclonal Antibody (GT1086)
LIN28A 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. 인간에 반응하며, 다양한 종에 예측 반응성 있음. 단백질 G 정제, PBS 용액 형태, 보존제 없음. 줄기세포 연구 및 iPS 세포 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT1086 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LIN28 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787094 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (98%), Rat (98%), Cat (96%), Pig (96%), Chicken (82%), Sheep (97%), Bovine (96%).
- Positive Control: NT2D1, human ESC, LIN28 transfected HeLa lysates.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Lin28 is a transcription factor first identified for its role in developmental timing in C. elegans. It plays a key role in stem cell biology and the generation of induced pluripotent stem (iPS) cells. Expression of Lin28, together with transcription factors POU5F1, Sox2, and NANOG, enables the reprogramming of somatic cells into iPS cells. Further studies have shown that iPS cells can be generated using expression plasmids encoding Lin28, Sox2, POU5F1, and c-Myc, avoiding viral vectors and improving safety for regenerative medicine applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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