ELK Biotechnology Recombinant Human B7-H3 (C-6His)
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| Product name: | Recombinant Human B7-H3 (C-6His) |
| Description: | Recombinant Human B7 Homolog 3 is produced by our Mammalian expression system and the target gene encoding Leu29-Thr461 is expressed with a 6His tag at the C-terminus. |
| Expression Host: | Human Cells |
| Accession: | Q5ZPR3 |
| Molecular weight: | 47.3 KDa |
| Apparent molecular weight: | 65-90 KDa, reducing conditions |
| Other names: | CD276; B7H34Ig-B7-H3; B7-H3; B7 homolog 3; CD276 antigen; CD276 molecule; Costimulatory molecule |
| FORMULATION: | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Purity: | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin: | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Biological activity: | Immobilized Human B7-H3-His(Cat#CK62) at 2μg/ml (100 μl/well) can bind Anti-Human B7-H3 mAb(Cat#NC055 ) The ED50 of Anti-Human B7-H3 mAb(Cat#NC055 ) is 7.98 ng/ml. |
| Redissolve: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Delivery condition: | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
| Background: | CD276, also known as B7-H3, is a member of the B7 superfamily with signature IgV and IgG regions in extracellular domains. It is a type I transmembrane protein and shares 20–27% amino acid identity with other B7 family members. B7-H3 is involved in the activation of T lymphocytes, and regulates murine bone formation. It is also reported that B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is expressed on T-cells, natural killer cells, and antigen presenting cells, as well as some non-immune cells, such as osteoblasts, fibroblasts, fibroblast-like synoviocytes and epithelial cells. High expression of B7-H3 in tumor vasculature also correlates with poor survival in patients, suggesting that it may play a role in tumor cell migration. |
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